Construct: shRNA TRCN0000229982

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012257.3-954s21c1
DNA Barcode:
CTATGGTTCTGATGGTCTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HBP1 (26959)
Gene Description:
HMG-box transcription factor 1
Transcript:
RefSeq NM_012257.3 (NON-CURRENT)
Match location:
Position 954 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229982)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGGTTCTGATGGTCTAAA
Hairpin Sequence:
5'-CCGG-CTATGGTTCTGATGGTCTAAA-CTCGAG-TTTAGACCATCAGAACCATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGGTTCTGATGGTCTAAACTCGAGTTTAGACCATCAGAACCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGGTTCTGATGGTCTAAACTCGAGTTTAGACCATCAGAACCATAG-3'

Other clones with same target sequence:

(none)