Construct: shRNA TRCN0000229999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020922.2-1620s21c1
DNA Barcode:
TGTCTATCAGGGACCTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNK3 (65267)
Gene Description:
WNK lysine deficient protein kinase 3
Transcript:
RefSeq NM_020922.2 (NON-CURRENT)
Match location:
Position 1620 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000229999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCTATCAGGGACCTATTAA
Hairpin Sequence:
5'-CCGG-TGTCTATCAGGGACCTATTAA-CTCGAG-TTAATAGGTCCCTGATAGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCTATCAGGGACCTATTAACTCGAGTTAATAGGTCCCTGATAGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCTATCAGGGACCTATTAACTCGAGTTAATAGGTCCCTGATAGACA-3'

Other clones with same target sequence:

  1. TRCN0000219673