Construct: shRNA TRCN0000230037

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003875.2-603s21c1
DNA Barcode:
GATCCAGCAATATTCACTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GMPS (8833)
Gene Description:
guanine monophosphate synthase
Transcript:
RefSeq NM_003875.2 (NON-CURRENT)
Match location:
Position 603 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230037)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCAGCAATATTCACTATT
Hairpin Sequence:
5'-CCGG-GATCCAGCAATATTCACTATT-CTCGAG-AATAGTGAATATTGCTGGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCAGCAATATTCACTATTCTCGAGAATAGTGAATATTGCTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCAGCAATATTCACTATTCTCGAGAATAGTGAATATTGCTGGATC-3'

Other clones with same target sequence:

(none)