Construct: shRNA TRCN0000230147

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004041.3-828s21c1
DNA Barcode:
TCTGGATAAGGAGATCTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARRB1 (408)
Gene Description:
arrestin beta 1
Transcript:
RefSeq NM_004041.3 (NON-CURRENT)
Match location:
Position 828 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230147)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGGATAAGGAGATCTATTA
Hairpin Sequence:
5'-CCGG-TCTGGATAAGGAGATCTATTA-CTCGAG-TAATAGATCTCCTTATCCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGGATAAGGAGATCTATTACTCGAGTAATAGATCTCCTTATCCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGGATAAGGAGATCTATTACTCGAGTAATAGATCTCCTTATCCAGA-3'

Other clones with same target sequence:

(none)