Construct: shRNA TRCN0000230157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007166.2-1931s21c1
DNA Barcode:
GGCAATCTTGGCATCGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PICALM (8301)
Gene Description:
phosphatidylinositol binding clathrin assembly protein
Transcript:
RefSeq NM_007166.2 (NON-CURRENT)
Match location:
Position 1931 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAATCTTGGCATCGGAAAT
Hairpin Sequence:
5'-CCGG-GGCAATCTTGGCATCGGAAAT-CTCGAG-ATTTCCGATGCCAAGATTGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAATCTTGGCATCGGAAATCTCGAGATTTCCGATGCCAAGATTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAATCTTGGCATCGGAAATCTCGAGATTTCCGATGCCAAGATTGCC-3'

Other clones with same target sequence:

(none)