Construct: shRNA TRCN0000230158

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007166.2-2154s21c1
DNA Barcode:
TGACGCAACCAACCTTAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PICALM (8301)
Gene Description:
phosphatidylinositol binding clathrin assembly protein
Transcript:
RefSeq NM_007166.2 (NON-CURRENT)
Match location:
Position 2154 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230158)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACGCAACCAACCTTAATAT
Hairpin Sequence:
5'-CCGG-TGACGCAACCAACCTTAATAT-CTCGAG-ATATTAAGGTTGGTTGCGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACGCAACCAACCTTAATATCTCGAGATATTAAGGTTGGTTGCGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACGCAACCAACCTTAATATCTCGAGATATTAAGGTTGGTTGCGTCA-3'

Other clones with same target sequence:

(none)