Construct: shRNA TRCN0000230166

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004287.3-1758s21c1
DNA Barcode:
TGTACTAATACCAGGTATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GOSR2 (9570)
Gene Description:
golgi SNAP receptor complex member 2
Transcript:
RefSeq NM_004287.3 (NON-CURRENT)
Match location:
Position 1758 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230166)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTACTAATACCAGGTATATT
Hairpin Sequence:
5'-CCGG-TGTACTAATACCAGGTATATT-CTCGAG-AATATACCTGGTATTAGTACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTACTAATACCAGGTATATTCTCGAGAATATACCTGGTATTAGTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTACTAATACCAGGTATATTCTCGAGAATATACCTGGTATTAGTACA-3'

Other clones with same target sequence:

(none)