Construct: shRNA TRCN0000230213

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014314.3-4326s21c1
DNA Barcode:
CCCAATACAAATGGATTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DDX58 (23586)
Gene Description:
DExD/H-box helicase 58
Transcript:
RefSeq NM_014314.3 (NON-CURRENT)
Match location:
Position 4326 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230213)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAATACAAATGGATTATTT
Hairpin Sequence:
5'-CCGG-CCCAATACAAATGGATTATTT-CTCGAG-AAATAATCCATTTGTATTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAATACAAATGGATTATTTCTCGAGAAATAATCCATTTGTATTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAATACAAATGGATTATTTCTCGAGAAATAATCCATTTGTATTGGG-3'

Other clones with same target sequence:

(none)