Construct: shRNA TRCN0000230270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020662.2-1167s21c1
DNA Barcode:
CTTTCGCTCTTTGGACTAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRS2 (57380)
Gene Description:
magnesium transporter MRS2
Transcript:
RefSeq NM_020662.2 (NON-CURRENT)
Match location:
Position 1167 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTCGCTCTTTGGACTAATG
Hairpin Sequence:
5'-CCGG-CTTTCGCTCTTTGGACTAATG-CTCGAG-CATTAGTCCAAAGAGCGAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTCGCTCTTTGGACTAATGCTCGAGCATTAGTCCAAAGAGCGAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTCGCTCTTTGGACTAATGCTCGAGCATTAGTCCAAAGAGCGAAAG-3'

Other clones with same target sequence:

(none)