Construct: shRNA TRCN0000230284

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001688.4-1279s21c1
DNA Barcode:
GTCTACTGAAGTTATAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATP5PB (515)
Gene Description:
ATP synthase peripheral stalk-membrane subunit b
Transcript:
RefSeq NM_001688.4 (NON-CURRENT)
Match location:
Position 1279 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230284)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTACTGAAGTTATAGTTTA
Hairpin Sequence:
5'-CCGG-GTCTACTGAAGTTATAGTTTA-CTCGAG-TAAACTATAACTTCAGTAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTACTGAAGTTATAGTTTACTCGAGTAAACTATAACTTCAGTAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTACTGAAGTTATAGTTTACTCGAGTAAACTATAACTTCAGTAGAC-3'

Other clones with same target sequence:

(none)