Construct: shRNA TRCN0000230347

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024077.3-2833s21c1
DNA Barcode:
ACTCAGATGTGCAGGTGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SECISBP2 (79048)
Gene Description:
SECIS binding protein 2
Transcript:
RefSeq NM_024077.3 (NON-CURRENT)
Match location:
Position 2833 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230347)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCAGATGTGCAGGTGTTAA
Hairpin Sequence:
5'-CCGG-ACTCAGATGTGCAGGTGTTAA-CTCGAG-TTAACACCTGCACATCTGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCAGATGTGCAGGTGTTAACTCGAGTTAACACCTGCACATCTGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCAGATGTGCAGGTGTTAACTCGAGTTAACACCTGCACATCTGAGT-3'

Other clones with same target sequence:

(none)