Construct: shRNA TRCN0000230501

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003760.3-3421s21c1
DNA Barcode:
CACGTATGGACCAGTACTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF4G3 (8672)
Gene Description:
eukaryotic translation initiation factor 4 gamma 3
Transcript:
RefSeq NM_003760.3 (NON-CURRENT)
Match location:
Position 3421 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230501)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGTATGGACCAGTACTTTA
Hairpin Sequence:
5'-CCGG-CACGTATGGACCAGTACTTTA-CTCGAG-TAAAGTACTGGTCCATACGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGTATGGACCAGTACTTTACTCGAGTAAAGTACTGGTCCATACGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGTATGGACCAGTACTTTACTCGAGTAAAGTACTGGTCCATACGTG-3'

Other clones with same target sequence:

(none)