Construct: shRNA TRCN0000230526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173500.3-2386s21c1
DNA Barcode:
AGCGACGCCCACAAGTCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TTBK2 (146057)
Gene Description:
tau tubulin kinase 2
Transcript:
RefSeq NM_173500.3 (NON-CURRENT)
Match location:
Position 2386 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCGACGCCCACAAGTCTAAT
Hairpin Sequence:
5'-CCGG-AGCGACGCCCACAAGTCTAAT-CTCGAG-ATTAGACTTGTGGGCGTCGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCGACGCCCACAAGTCTAATCTCGAGATTAGACTTGTGGGCGTCGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCGACGCCCACAAGTCTAATCTCGAGATTAGACTTGTGGGCGTCGCT-3'

Other clones with same target sequence:

(none)