Construct: shRNA TRCN0000230531

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080409.1-2859s21c1
DNA Barcode:
TAGCCATTTCTCACGCCTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF99 (7652)
Gene Description:
zinc finger protein 99
Transcript:
RefSeq NM_001080409.1 (NON-CURRENT)
Match location:
Position 2859 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230531)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAGCCATTTCTCACGCCTTAC
Hairpin Sequence:
5'-CCGG-TAGCCATTTCTCACGCCTTAC-CTCGAG-GTAAGGCGTGAGAAATGGCTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAGCCATTTCTCACGCCTTACCTCGAGGTAAGGCGTGAGAAATGGCTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAGCCATTTCTCACGCCTTACCTCGAGGTAAGGCGTGAGAAATGGCTA-3'

Other clones with same target sequence:

(none)