Construct: shRNA TRCN0000230550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144571.2-301s21c1
DNA Barcode:
TGATATTGCCAAGCTTCATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT6L (246175)
Gene Description:
CCR4-NOT transcription complex subunit 6 like
Transcript:
RefSeq NM_144571.2 (NON-CURRENT)
Match location:
Position 301 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATATTGCCAAGCTTCATAA
Hairpin Sequence:
5'-CCGG-TGATATTGCCAAGCTTCATAA-CTCGAG-TTATGAAGCTTGGCAATATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATATTGCCAAGCTTCATAACTCGAGTTATGAAGCTTGGCAATATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATATTGCCAAGCTTCATAACTCGAGTTATGAAGCTTGGCAATATCA-3'

Other clones with same target sequence:

(none)