Construct: shRNA TRCN0000230551

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144571.2-7978s21c1
DNA Barcode:
AGTCATTCTGTGACCTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CNOT6L (246175)
Gene Description:
CCR4-NOT transcription complex subunit 6 like
Transcript:
RefSeq NM_144571.2 (NON-CURRENT)
Match location:
Position 7978 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230551)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCATTCTGTGACCTATAAA
Hairpin Sequence:
5'-CCGG-AGTCATTCTGTGACCTATAAA-CTCGAG-TTTATAGGTCACAGAATGACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCATTCTGTGACCTATAAACTCGAGTTTATAGGTCACAGAATGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCATTCTGTGACCTATAAACTCGAGTTTATAGGTCACAGAATGACT-3'

Other clones with same target sequence:

(none)