Construct: shRNA TRCN0000230647

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001522.2-3642s21c1
DNA Barcode:
AGGCAATGGGAAAGCTCACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GUCY2F (2986)
Gene Description:
guanylate cyclase 2F, retinal
Transcript:
RefSeq NM_001522.2 (NON-CURRENT)
Match location:
Position 3642 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230647)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGCAATGGGAAAGCTCACTT
Hairpin Sequence:
5'-CCGG-AGGCAATGGGAAAGCTCACTT-CTCGAG-AAGTGAGCTTTCCCATTGCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGCAATGGGAAAGCTCACTTCTCGAGAAGTGAGCTTTCCCATTGCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGCAATGGGAAAGCTCACTTCTCGAGAAGTGAGCTTTCCCATTGCCT-3'

Other clones with same target sequence:

  1. TRCN0000000533