Construct: shRNA TRCN0000230775

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_194325.2-1866s21c1
DNA Barcode:
CTCGTACCTTGTACAACATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF30 (90075)
Gene Description:
zinc finger protein 30
Transcript:
RefSeq NM_194325.2 (NON-CURRENT)
Match location:
Position 1866 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230775)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCGTACCTTGTACAACATAG
Hairpin Sequence:
5'-CCGG-CTCGTACCTTGTACAACATAG-CTCGAG-CTATGTTGTACAAGGTACGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCGTACCTTGTACAACATAGCTCGAGCTATGTTGTACAAGGTACGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCGTACCTTGTACAACATAGCTCGAGCTATGTTGTACAAGGTACGAG-3'

Other clones with same target sequence:

(none)