Construct: shRNA TRCN0000230781

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024838.4-795s21c1
DNA Barcode:
GTACCTCTACTAGATCTAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THNSL1 (79896)
Gene Description:
threonine synthase like 1
Transcript:
RefSeq NM_024838.4 (NON-CURRENT)
Match location:
Position 795 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230781)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACCTCTACTAGATCTAATT
Hairpin Sequence:
5'-CCGG-GTACCTCTACTAGATCTAATT-CTCGAG-AATTAGATCTAGTAGAGGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACCTCTACTAGATCTAATTCTCGAGAATTAGATCTAGTAGAGGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACCTCTACTAGATCTAATTCTCGAGAATTAGATCTAGTAGAGGTAC-3'

Other clones with same target sequence:

(none)