Construct: shRNA TRCN0000230840

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003866.2-3674s21c1
DNA Barcode:
CTGAGGAAACATGGATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INPP4B (8821)
Gene Description:
inositol polyphosphate-4-phosphatase type II B
Transcript:
RefSeq NM_003866.2 (NON-CURRENT)
Match location:
Position 3674 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230840)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGGAAACATGGATCATTT
Hairpin Sequence:
5'-CCGG-CTGAGGAAACATGGATCATTT-CTCGAG-AAATGATCCATGTTTCCTCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGGAAACATGGATCATTTCTCGAGAAATGATCCATGTTTCCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGGAAACATGGATCATTTCTCGAGAAATGATCCATGTTTCCTCAG-3'

Other clones with same target sequence:

(none)