Construct: shRNA TRCN0000230937

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000807.2-1729s21c1
DNA Barcode:
TATGTACAGTCTGACTAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRA2 (2555)
Gene Description:
gamma-aminobutyric acid type A receptor alpha2 subunit
Transcript:
RefSeq NM_000807.2 (NON-CURRENT)
Match location:
Position 1729 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230937)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATGTACAGTCTGACTAATAA
Hairpin Sequence:
5'-CCGG-TATGTACAGTCTGACTAATAA-CTCGAG-TTATTAGTCAGACTGTACATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATGTACAGTCTGACTAATAACTCGAGTTATTAGTCAGACTGTACATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATGTACAGTCTGACTAATAACTCGAGTTATTAGTCAGACTGTACATA-3'

Other clones with same target sequence:

(none)