Construct: shRNA TRCN0000230941

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019041.5-2904s21c1
DNA Barcode:
TGGGATGAAGGCCCATAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MTRF1L (54516)
Gene Description:
mitochondrial translational release factor 1 like
Transcript:
RefSeq NM_019041.5 (NON-CURRENT)
Match location:
Position 2904 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230941)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGATGAAGGCCCATAATAA
Hairpin Sequence:
5'-CCGG-TGGGATGAAGGCCCATAATAA-CTCGAG-TTATTATGGGCCTTCATCCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGATGAAGGCCCATAATAACTCGAGTTATTATGGGCCTTCATCCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGATGAAGGCCCATAATAACTCGAGTTATTATGGGCCTTCATCCCA-3'

Other clones with same target sequence:

(none)