Construct: shRNA TRCN0000230965

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001132307.2-476s21c1
DNA Barcode:
TTTGTGATAACTGCGTAGAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC730077 (730077)
Gene Description:
hypothetical LOC730077
Transcript:
RefSeq XM_001132307.2 (NON-CURRENT)
Match location:
Position 476 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230965)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGTGATAACTGCGTAGAAC
Hairpin Sequence:
5'-CCGG-TTTGTGATAACTGCGTAGAAC-CTCGAG-GTTCTACGCAGTTATCACAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGTGATAACTGCGTAGAACCTCGAGGTTCTACGCAGTTATCACAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGTGATAACTGCGTAGAACCTCGAGGTTCTACGCAGTTATCACAAA-3'

Other clones with same target sequence:

(none)