Construct: shRNA TRCN0000230984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001720549.1-430s21c1
DNA Barcode:
TCCTTCGAGCAGGAGTTAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL7AP66 (388474)
Gene Description:
ribosomal protein L7a pseudogene 66
Transcript:
RefSeq XM_001720549.1 (NON-CURRENT)
Match location:
Position 430 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000230984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTTCGAGCAGGAGTTAATA
Hairpin Sequence:
5'-CCGG-TCCTTCGAGCAGGAGTTAATA-CTCGAG-TATTAACTCCTGCTCGAAGGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTTCGAGCAGGAGTTAATACTCGAGTATTAACTCCTGCTCGAAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTTCGAGCAGGAGTTAATACTCGAGTATTAACTCCTGCTCGAAGGA-3'

Other clones with same target sequence:

(none)