Construct: shRNA TRCN0000231008

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001719048.1-414s21c1
DNA Barcode:
GCTCAACAAGGCTTCGATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPL7P23 (648000)
Gene Description:
ribosomal protein L7 pseudogene 23
Transcript:
RefSeq XM_001719048.1 (NON-CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231008)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCAACAAGGCTTCGATTAA
Hairpin Sequence:
5'-CCGG-GCTCAACAAGGCTTCGATTAA-CTCGAG-TTAATCGAAGCCTTGTTGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCAACAAGGCTTCGATTAACTCGAGTTAATCGAAGCCTTGTTGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCAACAAGGCTTCGATTAACTCGAGTTAATCGAAGCCTTGTTGAGC-3'

Other clones with same target sequence:

(none)