Construct: shRNA TRCN0000231038

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012089.2-1879s21c1
DNA Barcode:
AGTGGCCTTCATCCGGAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCB10 (23456)
Gene Description:
ATP binding cassette subfamily B member 10
Transcript:
RefSeq NM_012089.2 (NON-CURRENT)
Match location:
Position 1879 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231038)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGGCCTTCATCCGGAATTT
Hairpin Sequence:
5'-CCGG-AGTGGCCTTCATCCGGAATTT-CTCGAG-AAATTCCGGATGAAGGCCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGGCCTTCATCCGGAATTTCTCGAGAAATTCCGGATGAAGGCCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGGCCTTCATCCGGAATTTCTCGAGAAATTCCGGATGAAGGCCACT-3'

Other clones with same target sequence:

(none)