Construct: shRNA TRCN0000231092

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003657.2-1532s21c1
DNA Barcode:
GTCACCAGTAGAGATTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BCAS1 (8537)
Gene Description:
breast carcinoma amplified sequence 1
Transcript:
RefSeq NM_003657.2 (NON-CURRENT)
Match location:
Position 1532 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231092)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCACCAGTAGAGATTATAAA
Hairpin Sequence:
5'-CCGG-GTCACCAGTAGAGATTATAAA-CTCGAG-TTTATAATCTCTACTGGTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCACCAGTAGAGATTATAAACTCGAGTTTATAATCTCTACTGGTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCACCAGTAGAGATTATAAACTCGAGTTTATAATCTCTACTGGTGAC-3'

Other clones with same target sequence:

(none)