Construct: shRNA TRCN0000231115

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006949.2-250s21c1
DNA Barcode:
CCCAGTCTGGAGGCCATTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STXBP2 (6813)
Gene Description:
syntaxin binding protein 2
Transcript:
RefSeq NM_006949.2 (NON-CURRENT)
Match location:
Position 250 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231115)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGTCTGGAGGCCATTTAT
Hairpin Sequence:
5'-CCGG-CCCAGTCTGGAGGCCATTTAT-CTCGAG-ATAAATGGCCTCCAGACTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGTCTGGAGGCCATTTATCTCGAGATAAATGGCCTCCAGACTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGTCTGGAGGCCATTTATCTCGAGATAAATGGCCTCCAGACTGGG-3'

Other clones with same target sequence:

(none)