Construct: shRNA TRCN0000231120

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174917.2-1627s21c1
DNA Barcode:
ACCCTCCGTGTTTCGAGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSF3 (197322)
Gene Description:
acyl-CoA synthetase family member 3
Transcript:
RefSeq NM_174917.2 (NON-CURRENT)
Match location:
Position 1627 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231120)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCCTCCGTGTTTCGAGAATA
Hairpin Sequence:
5'-CCGG-ACCCTCCGTGTTTCGAGAATA-CTCGAG-TATTCTCGAAACACGGAGGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCCTCCGTGTTTCGAGAATACTCGAGTATTCTCGAAACACGGAGGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCCTCCGTGTTTCGAGAATACTCGAGTATTCTCGAAACACGGAGGGT-3'

Other clones with same target sequence:

(none)