Construct: shRNA TRCN0000231122

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174917.2-2180s21c1
DNA Barcode:
CAAATCAGGTCACGTAGAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACSF3 (197322)
Gene Description:
acyl-CoA synthetase family member 3
Transcript:
RefSeq NM_174917.2 (NON-CURRENT)
Match location:
Position 2180 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231122)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAATCAGGTCACGTAGAATC
Hairpin Sequence:
5'-CCGG-CAAATCAGGTCACGTAGAATC-CTCGAG-GATTCTACGTGACCTGATTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAATCAGGTCACGTAGAATCCTCGAGGATTCTACGTGACCTGATTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAATCAGGTCACGTAGAATCCTCGAGGATTCTACGTGACCTGATTTG-3'

Other clones with same target sequence:

(none)