Construct: shRNA TRCN0000231337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001001171.2-2584s21c1
DNA Barcode:
CTGGAACAGGAGCGCTTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Trip11 (109181)
Gene Description:
thyroid hormone receptor interactor 11
Transcript:
RefSeq XM_001001171.2 (NON-CURRENT)
Match location:
Position 2584 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGAACAGGAGCGCTTAATT
Hairpin Sequence:
5'-CCGG-CTGGAACAGGAGCGCTTAATT-CTCGAG-AATTAAGCGCTCCTGTTCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGAACAGGAGCGCTTAATTCTCGAGAATTAAGCGCTCCTGTTCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGAACAGGAGCGCTTAATTCTCGAGAATTAAGCGCTCCTGTTCCAG-3'

Other clones with same target sequence:

(none)