Construct: shRNA TRCN0000231353

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001471678.1-525s21c1
DNA Barcode:
ATGAGCGTGGACCGCTACATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC100044222 (100044222)
Gene Description:
hypothetical protein LOC100044222
Transcript:
RefSeq XM_001471678.1 (NON-CURRENT)
Match location:
Position 525 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231353)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGAGCGTGGACCGCTACATT
Hairpin Sequence:
5'-CCGG-ATGAGCGTGGACCGCTACATT-CTCGAG-AATGTAGCGGTCCACGCTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGAGCGTGGACCGCTACATTCTCGAGAATGTAGCGGTCCACGCTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGAGCGTGGACCGCTACATTCTCGAGAATGTAGCGGTCCACGCTCAT-3'

Other clones with same target sequence:

(none)