Construct: shRNA TRCN0000231376

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007713.4-1881s21c1
DNA Barcode:
TTGAGTACTACCGTGGCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clk3 (102414)
Gene Description:
CDC-like kinase 3
Transcript:
RefSeq NM_007713.4 (CURRENT)
Match location:
Position 1881 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231376)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGAGTACTACCGTGGCTTTA
Hairpin Sequence:
5'-CCGG-TTGAGTACTACCGTGGCTTTA-CTCGAG-TAAAGCCACGGTAGTACTCAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGAGTACTACCGTGGCTTTACTCGAGTAAAGCCACGGTAGTACTCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGAGTACTACCGTGGCTTTACTCGAGTAAAGCCACGGTAGTACTCAA-3'

Other clones with same target sequence:

(none)