Construct: shRNA TRCN0000231418

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002819.3-193s21c1
DNA Barcode:
GCTTCTGCAGCAAACGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PTBP1 (5725)
Gene Description:
polypyrimidine tract binding protein 1
Transcript:
RefSeq NM_002819.3 (NON-CURRENT)
Match location:
Position 193 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231418)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTCTGCAGCAAACGGAAAT
Hairpin Sequence:
5'-CCGG-GCTTCTGCAGCAAACGGAAAT-CTCGAG-ATTTCCGTTTGCTGCAGAAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTCTGCAGCAAACGGAAATCTCGAGATTTCCGTTTGCTGCAGAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTCTGCAGCAAACGGAAATCTCGAGATTTCCGTTTGCTGCAGAAGC-3'

Other clones with same target sequence:

(none)