Construct: shRNA TRCN0000231461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004027.1-2650s21c1
DNA Barcode:
GAGAGTTTGGTGCGGTTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
INPP4A (3631)
Gene Description:
inositol polyphosphate-4-phosphatase type I A
Transcript:
RefSeq NM_004027.1 (NON-CURRENT)
Match location:
Position 2650 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGAGTTTGGTGCGGTTAAAT
Hairpin Sequence:
5'-CCGG-GAGAGTTTGGTGCGGTTAAAT-CTCGAG-ATTTAACCGCACCAAACTCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGAGTTTGGTGCGGTTAAATCTCGAGATTTAACCGCACCAAACTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGAGTTTGGTGCGGTTAAATCTCGAGATTTAACCGCACCAAACTCTC-3'

Other clones with same target sequence:

(none)