Construct: shRNA TRCN0000231481

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003557.1-2450s21c1
DNA Barcode:
ACAGACTAGCTGGCACATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIP5K1A (8394)
Gene Description:
phosphatidylinositol-4-phosphate 5-kinase type 1 alpha
Transcript:
RefSeq NM_003557.1 (NON-CURRENT)
Match location:
Position 2450 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231481)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGACTAGCTGGCACATTAT
Hairpin Sequence:
5'-CCGG-ACAGACTAGCTGGCACATTAT-CTCGAG-ATAATGTGCCAGCTAGTCTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGACTAGCTGGCACATTATCTCGAGATAATGTGCCAGCTAGTCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGACTAGCTGGCACATTATCTCGAGATAATGTGCCAGCTAGTCTGT-3'

Other clones with same target sequence:

(none)