Construct: shRNA TRCN0000231508

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014225.3-1615s21c1
DNA Barcode:
CTACGCTCTTCTGCATCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP2R1A (5518)
Gene Description:
protein phosphatase 2 scaffold subunit Aalpha
Transcript:
RefSeq NM_014225.3 (NON-CURRENT)
Match location:
Position 1615 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231508)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACGCTCTTCTGCATCAATG
Hairpin Sequence:
5'-CCGG-CTACGCTCTTCTGCATCAATG-CTCGAG-CATTGATGCAGAAGAGCGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACGCTCTTCTGCATCAATGCTCGAGCATTGATGCAGAAGAGCGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACGCTCTTCTGCATCAATGCTCGAGCATTGATGCAGAAGAGCGTAG-3'

Other clones with same target sequence:

(none)