Construct: shRNA TRCN0000231518

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015292.1-3485s21c1
DNA Barcode:
TTCACCTAACAGGCCCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ESYT1 (23344)
Gene Description:
extended synaptotagmin 1
Transcript:
RefSeq NM_015292.1 (NON-CURRENT)
Match location:
Position 3485 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231518)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCACCTAACAGGCCCATATT
Hairpin Sequence:
5'-CCGG-TTCACCTAACAGGCCCATATT-CTCGAG-AATATGGGCCTGTTAGGTGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCACCTAACAGGCCCATATTCTCGAGAATATGGGCCTGTTAGGTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCACCTAACAGGCCCATATTCTCGAGAATATGGGCCTGTTAGGTGAA-3'

Other clones with same target sequence:

(none)