Construct: shRNA TRCN0000231527

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006293.2-2755s21c1
DNA Barcode:
CCAGTGACTGTCGGTACATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TYRO3 (7301)
Gene Description:
TYRO3 protein tyrosine kinase
Transcript:
RefSeq NM_006293.2 (NON-CURRENT)
Match location:
Position 2755 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231527)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGACTGTCGGTACATAC
Hairpin Sequence:
5'-CCGG-CCAGTGACTGTCGGTACATAC-CTCGAG-GTATGTACCGACAGTCACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGACTGTCGGTACATACCTCGAGGTATGTACCGACAGTCACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGACTGTCGGTACATACCTCGAGGTATGTACCGACAGTCACTGG-3'

Other clones with same target sequence:

(none)