Construct: shRNA TRCN0000231573

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000625.3-3624s21c1
DNA Barcode:
GAAGCGCTATCACGAAGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOS2 (4843)
Gene Description:
nitric oxide synthase 2
Transcript:
RefSeq NM_000625.3 (NON-CURRENT)
Match location:
Position 3624 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231573)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAGCGCTATCACGAAGATAT
Hairpin Sequence:
5'-CCGG-GAAGCGCTATCACGAAGATAT-CTCGAG-ATATCTTCGTGATAGCGCTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAGCGCTATCACGAAGATATCTCGAGATATCTTCGTGATAGCGCTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAGCGCTATCACGAAGATATCTCGAGATATCTTCGTGATAGCGCTTC-3'

Other clones with same target sequence:

(none)