Construct: shRNA TRCN0000231593

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006313.1-2530s21c1
DNA Barcode:
GCAGGTCCTTGCCGCTATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
USP15 (9958)
Gene Description:
ubiquitin specific peptidase 15
Transcript:
RefSeq NM_006313.1 (NON-CURRENT)
Match location:
Position 2530 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231593)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGTCCTTGCCGCTATAAT
Hairpin Sequence:
5'-CCGG-GCAGGTCCTTGCCGCTATAAT-CTCGAG-ATTATAGCGGCAAGGACCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGTCCTTGCCGCTATAATCTCGAGATTATAGCGGCAAGGACCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGTCCTTGCCGCTATAATCTCGAGATTATAGCGGCAAGGACCTGC-3'

Other clones with same target sequence:

(none)