Construct: shRNA TRCN0000231617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012118.2-1507s21c1
DNA Barcode:
TTTAATCACAGGAGTCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NOCT (25819)
Gene Description:
nocturnin
Transcript:
RefSeq NM_012118.2 (NON-CURRENT)
Match location:
Position 1507 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTAATCACAGGAGTCTATTT
Hairpin Sequence:
5'-CCGG-TTTAATCACAGGAGTCTATTT-CTCGAG-AAATAGACTCCTGTGATTAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTAATCACAGGAGTCTATTTCTCGAGAAATAGACTCCTGTGATTAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTAATCACAGGAGTCTATTTCTCGAGAAATAGACTCCTGTGATTAAA-3'

Other clones with same target sequence:

(none)