Construct: shRNA TRCN0000231638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030768.2-844s21c1
DNA Barcode:
GGCAATCTTGTGTCGTTATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ILKAP (80895)
Gene Description:
ILK associated serine/threonine phosphatase
Transcript:
RefSeq NM_030768.2 (NON-CURRENT)
Match location:
Position 844 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAATCTTGTGTCGTTATAA
Hairpin Sequence:
5'-CCGG-GGCAATCTTGTGTCGTTATAA-CTCGAG-TTATAACGACACAAGATTGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAATCTTGTGTCGTTATAACTCGAGTTATAACGACACAAGATTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAATCTTGTGTCGTTATAACTCGAGTTATAACGACACAAGATTGCC-3'

Other clones with same target sequence:

(none)