Construct: shRNA TRCN0000231828

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_205767.1-662s21c1
DNA Barcode:
CCTGGAATGCAGGCATCATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MICOS13 (125988)
Gene Description:
mitochondrial contact site and cristae organizing system subunit 13
Transcript:
RefSeq NM_205767.1 (NON-CURRENT)
Match location:
Position 662 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231828)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGGAATGCAGGCATCATGA
Hairpin Sequence:
5'-CCGG-CCTGGAATGCAGGCATCATGA-CTCGAG-TCATGATGCCTGCATTCCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGGAATGCAGGCATCATGACTCGAGTCATGATGCCTGCATTCCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGGAATGCAGGCATCATGACTCGAGTCATGATGCCTGCATTCCAGG-3'

Other clones with same target sequence:

(none)