Construct: shRNA TRCN0000231831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178830.2-1066s21c1
DNA Barcode:
AGCCGGAGTCCTTGTCTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C19orf47 (126526)
Gene Description:
chromosome 19 open reading frame 47
Transcript:
RefSeq NM_178830.2 (NON-CURRENT)
Match location:
Position 1066 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCCGGAGTCCTTGTCTAAAG
Hairpin Sequence:
5'-CCGG-AGCCGGAGTCCTTGTCTAAAG-CTCGAG-CTTTAGACAAGGACTCCGGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCCGGAGTCCTTGTCTAAAGCTCGAGCTTTAGACAAGGACTCCGGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCCGGAGTCCTTGTCTAAAGCTCGAGCTTTAGACAAGGACTCCGGCT-3'

Other clones with same target sequence:

(none)