Construct: shRNA TRCN0000231844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005469.2-546s21c1
DNA Barcode:
GACCCTCATTGACCAGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACOT8 (10005)
Gene Description:
acyl-CoA thioesterase 8
Transcript:
RefSeq NM_005469.2 (NON-CURRENT)
Match location:
Position 546 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCCTCATTGACCAGTATTT
Hairpin Sequence:
5'-CCGG-GACCCTCATTGACCAGTATTT-CTCGAG-AAATACTGGTCAATGAGGGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCCTCATTGACCAGTATTTCTCGAGAAATACTGGTCAATGAGGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCCTCATTGACCAGTATTTCTCGAGAAATACTGGTCAATGAGGGTC-3'

Other clones with same target sequence:

(none)