Construct: shRNA TRCN0000231862

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000810.2-2146s21c1
DNA Barcode:
GCGAGAAACAGAGATCATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRA5 (2558)
Gene Description:
gamma-aminobutyric acid type A receptor alpha5 subunit
Transcript:
RefSeq NM_000810.2 (NON-CURRENT)
Match location:
Position 2146 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231862)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGAAACAGAGATCATAAA
Hairpin Sequence:
5'-CCGG-GCGAGAAACAGAGATCATAAA-CTCGAG-TTTATGATCTCTGTTTCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGAAACAGAGATCATAAACTCGAGTTTATGATCTCTGTTTCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGAAACAGAGATCATAAACTCGAGTTTATGATCTCTGTTTCTCGC-3'

Other clones with same target sequence:

(none)