Construct: shRNA TRCN0000231891

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006763.2-1561s21c1
DNA Barcode:
GGTCATAGAGCTACCGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BTG2 (7832)
Gene Description:
BTG anti-proliferation factor 2
Transcript:
RefSeq NM_006763.2 (NON-CURRENT)
Match location:
Position 1561 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231891)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCATAGAGCTACCGTATTT
Hairpin Sequence:
5'-CCGG-GGTCATAGAGCTACCGTATTT-CTCGAG-AAATACGGTAGCTCTATGACC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCATAGAGCTACCGTATTTCTCGAGAAATACGGTAGCTCTATGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCATAGAGCTACCGTATTTCTCGAGAAATACGGTAGCTCTATGACC-3'

Other clones with same target sequence:

(none)