Construct: shRNA TRCN0000231970

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153326.1-824s21c1
DNA Barcode:
ACTTCAACAGTCGGCAGATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKR1A1 (10327)
Gene Description:
aldo-keto reductase family 1 member A1
Transcript:
RefSeq NM_153326.1 (NON-CURRENT)
Match location:
Position 824 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000231970)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTCAACAGTCGGCAGATTG
Hairpin Sequence:
5'-CCGG-ACTTCAACAGTCGGCAGATTG-CTCGAG-CAATCTGCCGACTGTTGAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTCAACAGTCGGCAGATTGCTCGAGCAATCTGCCGACTGTTGAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTCAACAGTCGGCAGATTGCTCGAGCAATCTGCCGACTGTTGAAGT-3'

Other clones with same target sequence:

(none)