Construct: shRNA TRCN0000232119

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002697.2-1670s21c1
DNA Barcode:
ACAACACAGCAACCGTGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POU2F1 (5451)
Gene Description:
POU class 2 homeobox 1
Transcript:
RefSeq NM_002697.2 (NON-CURRENT)
Match location:
Position 1670 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000232119)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAACACAGCAACCGTGATTT
Hairpin Sequence:
5'-CCGG-ACAACACAGCAACCGTGATTT-CTCGAG-AAATCACGGTTGCTGTGTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAACACAGCAACCGTGATTTCTCGAGAAATCACGGTTGCTGTGTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAACACAGCAACCGTGATTTCTCGAGAAATCACGGTTGCTGTGTTGT-3'

Other clones with same target sequence:

(none)